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Long-term follow-up studies are needed to assess durability of therapeutic effects and to identify any delayed adverse effects or complications that might not be apparent in shorter-term investigations

The total RNA from the animals of each group was pooled together and cDNA was prepared using reverse transcriptase enzyme (Thermo Fischer, USA

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Bregman employs minimally invasive and minimal incision surgical techniques that reduce tissue disruption, minimize scarring, and support faster recovery

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