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DNA constructs Homo sapiens codon-optimized genes encoding di-domain glycerol-3-phosphate dehydrogenases (GPDHs) from Dunaliella viridis (GenBank: ACD84644.1) , Dunaliella salina (GenBank: AAX56341.1) , Chlamydomonas reinhardtii (GenBank: XP_042919880.1), and Sphaeroforma arctica (GenBank: XP_014155909.1) with removed chloroplast targeting sequences (see Supplementary Figure S1) and added a C-terminal linker sequence with a FLAG-tag flanked by EcoRI and AgeI restriction sites were custom synthesized and subcloned into pUC57 vectors by GENEWIZ

Keeping a symptom diary noting energy levels, food intake, and dose timing helps distinguish medication-related fatigue from other causes

(2009) 1:a002899

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