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L-theanine may help reduce stress, and a reduction in stress and increased relaxation can lower the heart rate

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Total RNA was extracted from the hippocampal tissues using an RNA simple Total RNA kit (Tiangen Biotech Co., Ltd.), and the FastKing gDNA Dispelling RT SuperMix kit (Tiangen Biotech Co., Ltd.) was used to synthesize cDNA

Gutt CN, Encke J, Koninger J, Harnoss JC, Weigand K, Kipfmuller K, Schunter O, Gotze T, Golling MT, Menges M, Klar E, Feilhauer K, Zoller WG, Ridwelski K, Ackmann S, Baron A, Schon MR, Seitz HK, Daniel D, Stremmel W, Buchler MW (2013) Acute cholecystitis: early versus delayed cholecystectomy, a multicenter randomized trial (ACDC study, NCT00447304)

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