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MTT assay was conducted using a microplate reader (SpectraMax)

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However, antibiotic treatment eliminates the beneficial effects of GABA by depleting gut bacteria (163)

They concluded from the study that Western style diets especially those with added cholesterol and longer feeding are the closest match across metabolic, histological, and molecular layers and Choline-deficient or genetically driven models may help elucidate specific fibrosis mechanisms but do not recapitulate the full human disease spectrum (302)

Because of this, theyre typically discussed in the context of diabetes or weight management