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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader

The Certificate of Analysis confirms purity, verifies molecular identity, and carries a unique batch number for full traceability

Sublingual administration offers a middle ground, providing better absorption than oral routes while avoiding injection discomfort

Dogs were housed individually and were provided with dog bed and were maintained on Envigo 2025 diet and tap water and were on a 12 h light cycle