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later by L-NNA (20 mg/kg i.p.) without or with following administration of PTX (10 mg/kg i.p.), in animals subsequently exposed to 3.5 h of WRS

They fill with water and expand the stomach

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Other factors must be taken into consideration when evaluating these testing methods including molecule absorption and metabolism, gastrointestinal motility, concurrent medication administration, as well as blood volume and blood flow to the intestine