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3 3 3 cm 3 voxels were placed in the thalamus (midline centered) and right sensorimotor cortex (the hand-knob of the motor cortex was used for initial localization and then the voxel was centered between the precentral gyrus and postcentral gyrus

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Twenty-four hours after seeding, media was exchanged to 2 mL of DMEM +dFBS (or RPMI +dFBS /McCoys 5A +dFBS ) and 300 ng/mL of Dox (except 786-O lenti cell lines)

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