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After being incubated in 0.1 M Na borate at RT for 30 min, sections were blocked as described above and incubated at 4 C for 48 h with mouse anti-BrdU (1:1000

This ensures the pen is working correctly and removes any air bubbles

Studies on mice have shown that activation of androgen receptors by dihydrotestosterone in the brains of the tested animals led to the development of IR, whereas rodents deprived of these receptors did not develop this type of disorder

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