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GLP-1 receptor agonists can modulate immune responses by influencing the signaling of immune cells and changing the signaling environment from pro-inflammatory to anti-inflammatory

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The cell pellet from 1 L of bacterial culture was suspended in 20 mL 50 mM Na 2 HPO 4 pH 8.5, 20 mM Imidazole, 500 mM NaCl, 5 mM GSH, supplemented with protease inhibitor (cOmplete Mini, EDTA-free, Roche) and lysed by sonication