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DOX is a highly toxic molecule and induces drug resistance due to the overexpression of ABCB1 efflux pump and other related proteins 127

In particular, GH opposes the actions of insulin: it increases glucose production through gluconeogenesis and glycogenolysis in the liver and kidney, suppresses glucose uptake in adipose tissues, and is lipolytic

In the absence of NEM an interfering peak in correspondence of the GS-NEM peak eluting at 4.95 min r.t