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Contents: 100 g / 3.527 oz

10.1002/ijc.29214 6 BarcelosR

Monocyte adhesion was higher in the center compared to the edge of the well, particularly with TNF- treatment

Reverse Transcription-Quantitative Polymerase Chain Reaction The total RNA content was isolated from cells or the tumor using TRIzol reagent (Invitrogen), treated with DNase I (Thermo Fisher Scientific, Cat# EN0521), and then reverse transcribed into cDNA using a Maximal First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat# K1622)