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Its properties make it suitable for techniques such as HPLC, mass spectrometry, and other research-based analytical methods

OConnor JC, McCusker RH, Strle K, Johnson RW, Dantzer R, Kelley KW

Keep technique consistent: Dont change diluent volume or handling style halfway through a study unless the protocol itself changes

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Those most at risk include vegans and vegetarians, people with digestive conditions such as Crohn's disease, coeliac disease or IBS, those taking long-term metformin or proton pump inhibitors, older adults whose ability to absorb B12 through the gut declines naturally with age, those with pernicious anaemia, and anyone with a history of chronic alcohol use