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As depicted in Figure 3D, AICAR treatment significantly inhibited the phosphorylation of PARP-1 while it increased the ABCA1 protein level

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After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)

The modest upward trend in AKT mRNA expression in the STZR group suggests partial preservation of transcriptional capacity

In the Control group, normal ovarian morphology was observed, with well-preserved primordial (yellow arrowhead), primary, secondary, and Graafian follicles